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. Author manuscript; available in PMC: 2012 Nov 20.
Published in final edited form as: Dev Biol. 2011 Apr 3;354(1):134–142. doi: 10.1016/j.ydbio.2011.03.027

Fig. 4.

Fig. 4

Effect of ectopic Cdx4 on the expression of cardiac genes in differentiating murine ES cells. (A) Time-course analysis in samples collected from iCdx4 ES cells differentiating as EB with or without exposure to doxycycline during days 2.25 to 6 of development shows continuous suppression of Gata4, Nkx2.5 and Isl-1. Results are shown as fold expression levels relative to gene expression in undifferentiated ES cells. Shown are data from one representative experiment showing more pronounced effects. (B) Gene expression analysis in day 5 and day 6 iCdx4 EB previously differentiated in basic medium with or without exposure to doxycycline as indicated shows strong suppression of Nkx2.5 while only mild, non-significant effects were seen on expression of Isl-1 and Gata4. Shown are data on % gene expression relative to non-induced controls collected at the same time-points in at least two independent experiments.