Table 1.

Gene	Forward Primer	Reverse Primer	Annealing temperature
Cdx1	GTA AGA CCCGAACCA AGGAC	GGA ACCAGATCTTTA CCTGC	58°C
Cdx4	GAGGAAGTCAGAGCTGGCAGTTA	GGCTCTGCGATTCTGAAACC	60°C
Gata4	AGGGTGAGCCTGTATGTA ATGCCT	AGGACCTGCTGGCGTCTTAGATTT	63°C
Isl1	GCA AGGACA AGA AACGCAGCATCA	ACTGGGTTAGCCTGTAAACCACCA	63°C
Mesp1	TACGCAGAA ACAGCATCCCAGGAA	CCAGGTTTCTAGAAGAGCCAGCAT	63°C
Nkx2.5	CAA GTGCTCTCCTGCTTTCC	CCAGCTCCACTGCCTTCTG	60°C
MyoD1	ATCCCTAAGCGACACAGA ACAGGGAA	TGCAGTCGATCTCTCAAAGCACCT	63°C
Myogenin	ACAATCTGCACTCCCTTACGTCCA	TCTCAGTTGGGCATGGTTTCGTCT	63°C
GAPDH	AATGCATCCTGCACCACCAACTGCTT	AGTGATGGCATGGACTGTGGTCAT	64°C
Actin	TCTTGGGTATGGAATCCTGTGGCA	ACTCCTGCTTGCTGATCCACATCT	59°C