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. Author manuscript; available in PMC: 2012 Nov 20.
Published in final edited form as: Kidney Int. 2008 Oct 8;75(3):295–303. doi: 10.1038/ki.2008.505

Figure 4. Immunoelectron microscopy of pS269-AQP2 in the normal rat kidney.

Figure 4

(a) In normal Wistar rat kidney collecting duct principal cells, pS269-AQP2 (arrows) is observed in the apical plasma membrane domain (apm). At high magnification (inset), it is clear that all gold particles are associated with the membrane. (b) After dDAVP treatment (30 min), the number of gold particles increases in the apical plasma membrane domain (arrows), and at high magnification (inset), it is clear that all gold particles are associated with the membrane and no intracellular gold particles can be observed. (c) After dDAVP treatment, no gold particles are associated with the basolateral membrane. L, lateral membrane; N, nucleus.