Figure 6. Short-term vasopressin treatment increases abundance of pS269-AQP2 in vivo.
Immunoblots of inner medulla homogenates from Wistar rats and Brattleboro rats treated with dDAVP for 30 min. Each lane represents a sample from a different rat. Values are mean band densities (± s.e.) normalized such that the mean for the control rats is defined as 1. In Brattleboro rats, under control conditions pS269-AQP2 is not detectable, thus the mean band density value is arbitrary. Abundance of total AQP2 is not significantly changed after dDAVP treatment, whereas pS269-AQP2 abundance increases significantly. Immunoblotting using actin confirmed equal loading. *Significant change in mean band densities between groups.