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. 2012 Nov 20;7(11):e49966. doi: 10.1371/journal.pone.0049966

Figure 2. The role of the two QS systems in the regulation of selected genes.

Figure 2

The bapA (A), bclA (B), and aidA (C) promoter activities were assessed by means of transcriptional lacZ fusions in the H111 wild type strain and in the mutant defective in AHL and BDSF synthesis (ΔcepI rpfF Bc). The strains were grown to late exponential growth phase in LB Lennox broth in the absence or presence of signal molecules (200 nM C8-HSL; 10 µM BDSF) as indicated by+and - below each bar. Error bars indicate SEM, n = 3. * P<0.05, ** P<0.01, *** P<0.001 (t-test, two-tailed) compared to ΔcepI rpfF Bc without signalling molecule (ns, not significant) (D) Expression of BclB and AidA in the H111 wild type and the double mutant ΔcepI rpfFBc as assessed by Western Blot analysis. The strains were grown on plates in the presence or absence of signal molecules as indicated by+and - below each band.