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. 2012 Nov 20;7(11):e50028. doi: 10.1371/journal.pone.0050028

Figure 3. MMP inhibitory activity of PEGylated rhTIMP-1.

Figure 3

(A) Silver stained gel shows purified rhTIMP-1, PEG5K -TIMP-1, and PEG20K -TIMP-1. Reactions were carried out using 100× molar excess of mPEG-SCM-5K or 5× molar excess of mPEG-SCM-20K, and PEGylated protein species were purified by ion exchange chromatography. (B) Graph shows inhibition of MMP-3cd by 0.8 molar equivalent of rhTIMP-1, PEG5K -TIMP-1 or PEG20K -TIMP-1. Protein concentrations were calculated from absorbance measurements using predicted molar extinction coefficients. (C) Graph shows titration of a fixed concentration of full-length recombinant human MMP-9 by increasing molar equivalents of rhTIMP-1 and PEG20K-TIMP-1.