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. 2012 Sep 1;5(5):462–465. doi: 10.4161/cib.21136

graphic file with name cib-5-462-g2.jpg

Figure 2. Molecular verification by RT-PCR of the proper expression of the sw transgene in testis of males with (A-) and without Sdic (B+). The lane of the underlined B+ corresponds to males carrying the endogenous copy of sw but not the transgene. H2O, negative control (no cDNA was added); L, ladder. Primers and methods are as described.10