Figure 4. Dose titration analysis of lead antibodies comparing the affinity gains of antibodies from the biased library strategy vs. the standard optimization approach. For lineage ICM10064 (A), the most potent scFv antibodies from the NNS and TiAM approaches respectively, were NNS_64_1 and TiAM_64_1. In the ICM10088 lineage (B), the most potent scFv antibodies from the NNS and TiAM approaches, respectively, were NNS_88_1 and TiAM_88_1. Full dose titration curves, with standard error bars, are plotted and calculated IC₅₀ values are shown for all antibodies.