Figure 5. Sequence summary for variants which have undergone TiAM and NNS optimization of V_LCDR3 loops in antibodies ICM10064 and ICM10088. For lineage ICM10064 (A) and ICM10088 (B), the wild type V_LCDR3 loop sequence is shown at the top and for each variant the changes incorporated during the affinity maturation process are highlighted. Amino acids are colored according to side-chain properties, as detailed in the legend, and the improvement in affinity measured for each individual variant is recorded.