Table 1. Summary of anti-HB-EGF mAb generation by mouse strain and immunogen.
| Immunization | Total | HB-EGF binder | Neutralizing mAb |
|---|---|---|---|
| KLH/BALB/ca |
17,952 |
1,018 (5.7%) |
19 (0.11%) |
| KLH/sHB-EGF/BALB/cb |
2,640 |
207 (7.8%) |
24 (0.91%) |
| KLH/cell/BALB/cc |
5,280 |
589 (11.2%) |
33 (0.63%) |
| KLH/C57BLd |
1,670 |
30 (1.8%) |
1 (0.06%) |
| KLH/C3He |
2,640 |
210 (8.0%) |
2 (0.08%) |
| KLH/CD1f |
5,720 |
754 (13.2%) |
41 (0.72%) |
| BSA/CD1g |
4,576 |
529 (11.6%) |
90 (1.97%) |
| Total | 40,478 | 3,337 (8.2%) | 210 (0.52%) |
The number of hybridoma clones of mAb for each immunization is shown. The production rates are noted in parentheses. a-cBALB/c immunization with aKLH-conjugated sHB-EGF; bthe KLH-conjugate and a final boost of sHB-EGF; cco-immunization of the KLH-conjugate and proHB-EGF-expressing cells; dC57BL immunization with the KLH-conjugate; eC3H immunization with the KLH-conjugate; f,gCD1 immunization with fthe KLH-conjugate; gBSA-conjugated sHB-EGF.