Table 2. Summary of physicochemical characteristics of unstressed and stressed IgG formulations.
| Frag | Aggregates |
Conform. changes |
Hydroph | Covalent bonds |
Epitope damage |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Olig | Submicron | Micron | Second | Tertiary | |||||||
|
Unst |
- |
- |
- |
- |
- |
- |
- |
- |
- |
||
|
FT |
- |
- |
+ |
++ |
+ |
+ |
- |
- |
- |
||
|
pH shift |
- |
++ |
++ |
+++ |
+ |
++ |
++ |
- |
- |
||
|
Heat |
- |
+++ |
+++ |
+ |
+ |
+ |
+++ |
++ |
- |
||
|
Shake |
- |
- |
+ |
+++ |
+ |
N/A |
+++ |
++ |
- |
||
| Met Ox | ++ | + | + | + | + | ++ | + | - | - | ||
Abbreviations: Unst, unstressed; FT, freeze-thawed; Met Ox, metal-catalyzed oxidized; Frag, fragments; Olig, oligomers; Conform, conformational; Second, secondary; Hydroph, hydrophobicity; N/A, not available (see text). Explanation of used symbols: negligible (-); mild (+); considerable (++); extensive (+++).