Table 1. Oligonucleotide primer sequences for RT-PCR and real -time quantitative RT-PCR.
Gene | Acc.No. | Species | Oligonucleotide sequence (5′-3′) | Size (bp) |
Atrap | NM_009642 | mouse | sense; CAGCTTGGCCCTTGTTCTCCAG antisense; CATCCTCAGCTTGCTGCTGAAG | 194 |
At2r1a | NM_177322 | mouse | sense; GCTGCTCTCCCGGACTTAA antisense; CGGAGGCGAGACTTCATTG | 565 |
At2r1b | NM_175086 | mouse | sense; GGGAGTAACAGAGACCAGACAAG antisense; CGGAGGCGAGACTTCATTG | 565 |
Actb | NM_007393 | mouse | sense; GTGGGGCGCCCCAGGCACCA antisense; CTCTTTGATGTCACGCACGATT | 540 |
Atrap | XM_003127573 | porcine | sense; GTCGACGCCATAAGTATGTTT antisense; CGGTACATGTGGTAGACGAG | 182 |
At2r1 | ENSSSCG00000011695 | porcine | sense; TATTTGGGAACAGTTTGGTG antisense; GAAACTGACACTGGCTGAAG | 203 |
GAPDH | NM_007393 | porcine | sense; GACAACTTCGGCATCGTG antisense; GCTCAGTGTAGCCCAGGAT | 340 |
TRPV2 | XM_003132023 | porcine | sense; ATCATCGCCTTTCACTGCC antisense; CAAAGTAGCTGTCCACGAAG | 343 |