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. 2012 Oct 5;19(1):86. doi: 10.1186/1423-0127-19-86

Figure 2.

Figure 2

Zerumbone evoked caspase-3 activation in GBM cells. (A) GBM8401 cells were pretreated with DMSO or zVAD-fmk (50 or 100 μM) for 30 min before the addition of zerumbone (50 μM) for another 24 h. Cell viability was then determined by the MTT assay. *p < 0.05, compared with the group treated with zerumbone alone. Cells were treated with DMSO or zerumbone (50 μM) for indicated time intervals. Protein levels of procaspase-3 and caspase-3 (B) and PARP (C) were then determined by immunoblotting. Typical traces, representive of data from 3 independent experiments with similar results, are shown.