Table 1.
Primer Sequences and the Expected Amplicon Sizes for the Multiplex Polymerase Chain Reaction Employed in the Detection of Diarrheagenic Escherichia coli
| Pathogen | Primer | Target Gene | Primer Sequence (5′-3′) | Amplicon (bp) |
|---|---|---|---|---|
| ETEC | LT-F | elt | CACACGGAGCTCCTCAGTC | 508 |
| LT-R | CCCCCAGCCTAGCTTAGTTT | |||
| ST-F | est | GCTAAACCAGTAG/AGGTCTTCAAAA | 147 | |
| ST-R | CCCGGTACAG/AGCAGGATTACAACA | |||
| EPEC | BFPA-F | bfpA | GGAAGTCAAATTCATGGGGG | 367 |
| BFPA-R | GGAATCAGACGCAGACTGGT | |||
| EAE-F | eae | CCCGAATTCGGCACAAGCATAAGC | 881 | |
| EAE-R | CCCGGATCCGTCTCGCCAGTATTCG | |||
| EAEC | CVD432F | aatA | CTGGCGAAAGACTGTATCAT | 630 |
| CVD432R | CAATGTATAGAAATCCGCTGTT | |||
| AAIC F | aaiC | ATTGTCCTCAGGCATTTCAC | 215 | |
| AAIC R | ACGACACCCCTGATAAACAA |
Abbreviations: EAEC, enteroaggregative Escherichia coli; EPEC, enteropathogenic E. coli; ETEC, enterotoxigenic E. coli.