Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Components of the Multiplex Polymerase Chain Reaction
| 1 × polymerase chain reaction | |
| 2 × GoTaq Green Master Mix | 15.0 μL |
| 20 μM bfpA-F | 1.0 μL |
| 20 μM bfpA-R | 1.0 μL |
| 20 μM ehxA-F | 1.0 μL |
| 20 μM ehxA-R | 1.0 μL |
| 20 μM eae-F | 1.0 μL |
| 20 μM eae-R | 1.0 μL |
| 20 μM stx1-F | 0.5 μL |
| 20 μM stx1-R | 0.5 μL |
| 20 μM stx2-F | 0.5 μL |
| 20 μM stx2-R | 0.5 μL |
| DNA template | 2.0 μL |
| Total volume | 25.0 μL |
