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Primer Sequences and the Expected Amplicon Sizes for the Multiplex Polymerase Chain Reaction
| Primer | Sequence (5′-3′) | TargetGene | Amplicon(bp) |
|---|---|---|---|
| eae-F | GACCCGGCACAAGCATAAGC | eae | 384 |
| eae-R | CCACCTGCAGCAACAAGAGG | ||
| ehxA-F | GCATCATCAAGCGTACGTTCC | ehxA | 534 |
| ehxA-R | AATGAGCCAAGCTGGTTAAGCT | ||
| stx1-F | ATAAATCGCCATTCGTTGACTAC | stx1 | 180 |
| stx1-R | AGAACGCCCACTGAGATCATC | ||
| stx2-F | GGCACTGTCTGAAACTGCTCC | stx2 | 255 |
| stx2-R | TCGCCAGTTATCTGACATTCTG | ||
| bfpA-F | GGAAGTCAAATTCATGGGGG | bfpA | 300 |
| bfpA-R | GGAATCAGACGCAGACTGGT |
