Table 5.
Primer Sequences and the Expected Amplicon Sizes for the Multiplex Polymerase Chain Reaction Used in the Detection of RNA Viruses
Pathogen | Primer | Primer Sequence (5′-3′) | Amplicon (bp) |
---|---|---|---|
Norovirus GI | G1SKR | CCAACCCARCCATTRTACA | 330 |
G1SKF | CTGCCCGAATTYGTAAATGA | ||
Norovirus GII | G2SKR | CCRCCNGCATRHCCRTTRTACAT | 387 |
COG2F | CARGARBCNATGTTYAGRTGGATGAG | ||
Sapovirus | SLV5749 | CGGRCYTCAAAVSTACCBCCCCA | 434 |
SLV5317 | CTCGCCACCTACRAWGCBTGGTT | ||
Astrovirus | 82b | GTGAGCCACCAGCCATCCCT | 719 |
PreCAP1 | GGACTGCAAAGCAGCTTCGTG | ||
Cog2R | TCGACGCCATCTTCATTCACA |