Table 5.
Primer Sequences and the Expected Amplicon Sizes for the Multiplex Polymerase Chain Reaction Used in the Detection of RNA Viruses
| Pathogen | Primer | Primer Sequence (5′-3′) | Amplicon (bp) |
|---|---|---|---|
| Norovirus GI | G1SKR | CCAACCCARCCATTRTACA | 330 |
| G1SKF | CTGCCCGAATTYGTAAATGA | ||
| Norovirus GII | G2SKR | CCRCCNGCATRHCCRTTRTACAT | 387 |
| COG2F | CARGARBCNATGTTYAGRTGGATGAG | ||
| Sapovirus | SLV5749 | CGGRCYTCAAAVSTACCBCCCCA | 434 |
| SLV5317 | CTCGCCACCTACRAWGCBTGGTT | ||
| Astrovirus | 82b | GTGAGCCACCAGCCATCCCT | 719 |
| PreCAP1 | GGACTGCAAAGCAGCTTCGTG | ||
| Cog2R | TCGACGCCATCTTCATTCACA |