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. 2012 Nov 20;7(11):e50201. doi: 10.1371/journal.pone.0050201

Figure 4. Colony morphology and α-(1,3)-glucan immunostaining.

Figure 4

(p) and (u) H. capsulatum amy1-null mutant complemented with P. brasiliensis AMY1 under the CBP1 promoter (pEC87) or AMY1 promoter (pEC90), respectively, is able to restore the rough phenotype exhibited by (a) the parental strain, as well as its α-(1,3)-glucan content and distribution. The smooth colony morphology correlates with loss of α-(1,3)-glucan from yeast cell walls as shown by immunofluorescence of (f) ags1-Δ mutant yeasts or (k) amy1-Δ mutant yeasts.