Table 1. Fungal strains and plasmids used in this study.
Fungal strains | Genotype or description | Source or reference |
P. brasiliensis IVIC Pb73 | Phylogenetic group PS3† | ATCC 32071 |
H. capsulatum WU8 | G186A ura5-Δ32 | [23] |
H. capsulatum WU11 | G186A ura5-Δ31 ags1-Δ3::hph | [23] |
H. capsulatum WU8 | G186A ura5-Δ31 amy1-Δ1::hph | [23] |
Plasmids | ||
pCR41 | PaURA5 PCBP1 | [11] |
pEC87 | PaURA5 PCBP1-PbAMY1 | This study |
pEC90 | PaURA5 PAMY1-PbAMY1 | This study |
pQE30Xa | E. coli specific vector for high.level expression of N-terminal 6xHis-tagged proteins. | Qiagen, Germany |
pQE30Xa-AMY | pQE30Xa containing PbAMY1 cDNA | This study |
hph, hygromycin phosphotransferase (hygromycin resistance).
PaURA5, Podospora anserina URA5 gene.
PbAMY1, P. brasiliensis AMY1 gene.
PCBP1, 889 bp upstream of the H. capsulatum CBP1 gene.
PAMY1, 1.950 bp upstream of the P. brasiliensis AMY1 gene.
Defined by [2].