Table 3. Quantification of α-(1,3)-glucan in H. capsulatum yeast cells by anthrone assay.
Strain | Relative glucosepresent in F2 (%) |
Parent+vector | 100±3.34 |
ags1-Δ+vector | 2.69±0.51 |
amy1-Δ+vector | 13.36±1.31 |
amy1-Δ+pEC87 | 75.38±1.15 |
amy1-Δ+pEC90 | 72.11±1.78 |
Samples were normalized by preparing a fraction 2 aqueous solution (1 µg ul−1).
Data represent the average from triplicate samples ± standard deviation.