Figure 5.

Accumulation of tasiRNAs in response to OS-elicitation. (A) Abundance of tasiRNA3, which is a target of the OS-inducible Nat-miR390, significantly increased in OS-elicited plant compared to control (CTRL) and W+W treatment. Abundance of tasiRNA4, which is regulated by the wound-inducible Nat-miR828, was significantly increased by wounding. (B) Accumulation of JA-independent tasiRNA3 and JA-dependent tasiRNA4 at 1 h and 5 h after OS-elicitation, respectively. For each sample, one leaf on the rosette of a 32-day-old plant was treated with wounding plus oral secretions of the larvae of the specialist herbivore M. sexta (W+OS) and harvested 1 h or 5 h post treatment. Untreated plants served as control. Shown are mean (± SE) levels of three replicates. Letters indicate significant differences (P < 0.05) in Fisher’s PLSD test following an ANOVA.