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VLPs stored in 15% trehalose at −70°C and subjected to different freeze-thaw cycles.A) 0 freeze-thaw cycles. B) 1 freeze-thaw cycle after 1 month. C) 2 freeze thaw cycles after 3 months; white arrows indicate electron dense areas. D) 3 freeze-thaw cycles after 6 months. Scale bars = 500 nm.
