Table 1.
Summary of morphological observations of VLPs under different storage conditions
|
Storage time (months) | |||||
|---|---|---|---|---|---|
| Formulation medium | Temperature | 1 | 3 | 6 | 12 |
| PBS |
4°C |
√ |
× |
nd |
nd |
| |
-20°C |
± |
× |
nd |
nd |
| |
-70°C |
± |
× |
nd |
nd |
| 5% Trehalose |
4°C |
× |
nd |
nd |
nd |
| |
-20°C |
± |
× |
nd |
nd |
| |
-70°C |
× |
nd |
nd |
nd |
| 15% Trehalose |
4°C |
√ |
× |
nd |
nd |
| |
-20°C |
√ |
√ |
√ |
√ |
| |
-70°C |
√ |
√ |
√ |
√ |
| 5% Sucrose |
4°C |
× |
nd |
nd |
nd |
| |
-20°C |
× |
nd |
nd |
nd |
| |
-70°C |
× |
nd |
nd |
nd |
| 15% Sucrose |
4°C |
√ |
× |
nd |
nd |
| |
-20°C |
√ |
± |
× |
nd |
| |
-70 |
√ |
√ |
× |
nd |
| 5% Sorbitol |
4°C |
nd |
nd |
nd |
nd |
| |
-20°C |
± |
× |
nd |
nd |
| |
-70°C |
± |
× |
nd |
nd |
| 15% Sorbitol |
4°C |
4°C |
nd |
nd |
nd |
| |
-20°C |
√ |
× |
nd |
nd |
| 70°C | √ | ± | × | nd | |
√: the formulated VLP micrograph is identical to that of the fresh batch.
±: 50% or more of the formulated VLPs in the micrograph have changed shape.
×: 100% of the formulated VLPs have changed shape.
nd: not determined.