Table 1.
Receptor specificity determination assay results of five subtypes of avian influenza viruses with and without α 2, 3-sialidase-enzyme treatment of RBCs
| No. | Virus strain | HA titer with enzyme treated 1% goose RBCs | HA titer with untreated 1% goose RBCs | HA titer with enzyme treated 0.5% turkey RBCs | HA titer with untreated 0.5% turkey RBCs | Receptor specificity Avian / mammalian | Amino acid present at position 221 |
|---|---|---|---|---|---|---|---|
| 1 |
A/chicken/India/NIV33487/06-RG-2008 (H5N1) |
No titer |
512 |
No titer |
512 |
SA α 2,3 Gal –Avian |
S |
| 2 |
A/chicken/India/WB-NIV2665/2008 (H5N1) |
16 |
16 |
16 |
16 |
SA α 2,3 Gal and α 2, 6 Gal- Avian and Mammalian |
P |
| 3 |
A/chicken/India/WB-NIV2670/2008 (H5N1) |
No titer |
32 |
No titer |
128 |
SA α 2,3 Gal–Avian |
P |
| 4 |
A/chicken/Manipur/NIV9743/2007 (H5N1) |
No titer |
8 |
No titer |
24 |
SA α 2,3 Gal–Avian |
S |
| 5 |
A/chicken/India/WB-NIV2811/2008 (H5N1) |
No titer |
24 |
No titer |
256 |
SA α 2,3 Gal–Avian |
S |
| 6 |
A/chicken/India/AS-NIV15983/2008 (H5N1) |
8 |
192 |
16 |
512 |
SA α 2,3 Gal and α 2, 6 Gal-Avian and Mammalian |
S |
| 7 |
A/duck/India/TR-NIV4396/2008 (H5N1) |
No titer |
32 |
No titer |
24 |
SA α 2,3 Gal–Avian |
S |
| 8 |
A/chicken/India-WB-NIV82853/2008 (H5N1) |
No titer |
16 |
No titer |
768 |
SA α 2,3 Gal–Avian |
S |
| 9 |
A/chicken/India/AS-NIV15618/2008 (H5N1) |
No titer |
48 |
No titer |
32 |
SA α 2,3 Gal–Avian |
S |
| 10 |
A/chicken/India/AS-NIV16717/2008 (H5N1) |
No titer |
16 |
No titer |
16 |
SA α 2,3 Gal–Avian |
S |
| 11 |
A/chicken/Pune/099321/2009 (H9N2) |
512 |
1024 |
512 |
1024 |
SA α 2,3 Gal and α 2, 6 Gal- Avian and Mammalian |
P |
| 12 |
A/aquatic bird/India/NIV-17095/2007 (H11N1) |
1024 |
1024 |
512 |
1024 |
SA α 2,3 Gal and α 2, 6 Gal- Avian and Mammalian |
P |
| 13 |
A/Duck/Nabagram-WB/101018/2009 (H4N6) |
No titer |
512 |
No titer |
128 |
SA α 2,3 Gal–Avian |
P |
| 14 | A/chicken/Italy/1067/1999 (H7N1) | No titer | 512 | No titer | 512 | SA α 2,3 Gal–Avian | P |