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. 2012 Nov 20;7(11):e48560. doi: 10.1371/journal.pone.0048560

Figure 2. Roscovitine enhances STAT3 activation and does not block TGF-β signaling.

Figure 2

A. MRC-5 and NL57 human fibroblasts, as well as primary fibroblasts from a scleroderma patient (SSc) or an unaffected twin (NL) were incubated overnight in serum-free medium and stimulated for 45 minutes with DMSO vehicle (D), roscovitine (R, 6.25 µg/mL), TGF-β (T, 10 ng/mL), or IL-6 (6, 50 ng/mL) alone or in combination as indicated. Cells were directly lysed, and proteins fractionated and blotted for phosphorylated ERK (p-ERK), STAT3 (p-STAT3), or SMAD3 (p-SMAD3) and in parallel blots for total STAT3 or SMAD3. B. SMAD3 activation in serum-starved MRC-5 cells exposed to TGF-β without (−) or with (+) roscovitine for up to 60 minutes as indicated in 2 parallel experiments.