Table 1.
Similarity values of rumen methanogens from yak and cattle from Qinghai-Tibetan Plateau, China
|
Yak |
Cattle |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 16S Sequence | Clonesa | OTU# | Nearest Taxon | % Seq ID | 16S Sequence | Clonesa | OTU# | Nearest Taxon | % Seq ID |
| QTPYAK1 |
5 |
74 |
Mms. luminyensis |
88.2 |
QTPC1 |
2 |
82 |
Mbb. millerae |
98.6 |
| QTPYAK2 |
1 |
74 |
Mms. luminyensis |
88.1 |
QTPC2 |
1 |
82 |
Mbb. millerae |
99.0 |
| QTPYAK3 |
1 |
82 |
Mbb. millerae |
97.9 |
QTPC3 |
3 |
82 |
Mbb. millerae |
98.2 |
| QTPYAK4 |
1 |
82 |
Mbb. millerae |
98.1 |
QTPC4 |
4 |
49 |
Mms. luminyensis |
87.9 |
| QTPYAK5 |
1 |
82 |
Mbb. millerae |
97.6 |
QTPC5 |
1 |
63 |
Mms. luminyensis |
88.4 |
| QTPYAK6 |
1 |
82 |
Mbb. millerae |
98.3 |
QTPC6 |
4 |
49 |
Mms. luminyensis |
87.8 |
| QTPYAK7 |
2 |
82 |
Mbb. millerae |
98.1 |
QTPC7 |
1 |
33 |
Mms. luminyensis |
87.8 |
| QTPYAK8 |
1 |
84 |
Mbb. millerae |
97.1 |
QTPC8 |
1 |
82 |
Mbb. millerae |
99.1 |
| QTPYAK9 |
1 |
82 |
Mbb. millerae |
98.0 |
QTPC9 |
2 |
82 |
Mbb. gottschalkii |
97.6 |
| QTPYAK10 |
1 |
82 |
Mbb. millerae |
98.2 |
QTPC10 |
1 |
82 |
Mbb. millerae |
98.3 |
| QTPYAK11 |
1 |
83 |
Mbb. millerae |
97.7 |
QTPC11 |
1 |
82 |
Mbb. millerae |
98.3 |
| QTPYAK12 |
1 |
89 |
Mbb. smithii |
96.3 |
QTPC12 |
1 |
82 |
Mbb. millerae |
97.7 |
| QTPYAK13 |
1 |
50 |
Mms. luminyensis |
87.9 |
QTPC13 |
1 |
82 |
Mbb. millerae |
98.4 |
| QTPYAK14 |
2 |
51 |
Mms. luminyensis |
88.8 |
QTPC14 |
1 |
82 |
Mbb. millerae |
98.7 |
| QTPYAK15 |
2 |
36 |
Mms. luminyensis |
87.1 |
QTPC15 |
1 |
82 |
Mbb. gottschalkii |
98.4 |
| QTPYAK16 |
1 |
52 |
Mms. luminyensis |
87.8 |
QTPC16 |
1 |
10 |
Mms. luminyensis |
87.1 |
| QTPYAK17 |
3 |
49 |
Mms. luminyensis |
88.2 |
QTPC17 |
3 |
82 |
Mbb. millerae |
98.0 |
| QTPYAK18 |
1 |
53 |
Mms. luminyensis |
88.0 |
QTPC18 |
1 |
82 |
Mbb. millerae |
97.9 |
| QTPYAK19 |
1 |
16 |
Mms. luminyensis |
87.0 |
QTPC19 |
2 |
82 |
Mbb. millerae |
97.9 |
| QTPYAK20 |
1 |
68 |
Mms. luminyensis |
87.4 |
QTPC20 |
1 |
82 |
Mbb. millerae |
98.3 |
| QTPYAK21 |
1 |
4 |
Mms. luminyensis |
88.0 |
QTPC21 |
1 |
82 |
Mbb. millerae |
98.5 |
| QTPYAK22 |
2 |
49 |
Mms. luminyensis |
88.1 |
QTPC22 |
1 |
82 |
Mbb. millerae |
98.4 |
| QTPYAK23 |
2 |
49 |
Mms. luminyensis |
88.1 |
QTPC23 |
2 |
82 |
Mbb. millerae |
97.7 |
| QTPYAK24 |
2 |
61 |
Mms. luminyensis |
88.4 |
QTPC24 |
1 |
82 |
Mbb. millerae |
98.3 |
| QTPYAK25 |
1 |
62 |
Mms. luminyensis |
88.6 |
QTPC25 |
2 |
82 |
Mbb. millerae |
98.1 |
| QTPYAK26 |
4 |
49 |
Mms. luminyensis |
88.0 |
QTPC26 |
2 |
82 |
Mbb. millerae |
97.9 |
| QTPYAK27 |
1 |
49 |
Mms. luminyensis |
87.8 |
QTPC27 |
1 |
86 |
Mbb. smithii |
96.8 |
| QTPYAK28 |
1 |
49 |
Mms. luminyensis |
88.5 |
QTPC28 |
1 |
49 |
Mms. luminyensis |
87.9 |
| QTPYAK29 |
1 |
49 |
Mms. luminyensis |
87.8 |
QTPC29 |
2 |
28 |
Mms. luminyensis |
86.8 |
| QTPYAK30 |
2 |
85 |
Mbb. smithii |
97.5 |
QTPC30 |
6 |
80 |
Mmb. mobile |
99.7 |
| QTPYAK31 |
2 |
82 |
Mbb. millerae |
98.3 |
QTPC31 |
1 |
80 |
Mmb. mobile |
99.7 |
| QTPYAK32 |
3 |
88 |
Mbb. millerae |
97.0 |
QTPC32 |
1 |
80 |
Mmb. mobile |
99.4 |
| QTPYAK33 |
1 |
90 |
Mbb. millerae |
97.0 |
QTPC33 |
3 |
80 |
Mmb. mobile |
99.5 |
| QTPYAK34 |
1 |
70 |
Mms. luminyensis |
88.5 |
QTPC34 |
2 |
80 |
Mmb. mobile |
99.5 |
| QTPYAK35 |
1 |
70 |
Mms. luminyensis |
88.4 |
QTPC35 |
7 |
80 |
Mmb. mobile |
99.8 |
| QTPYAK36 |
1 |
70 |
Mms. luminyensis |
88.4 |
QTPC36 |
4 |
70 |
Mms. luminyensis |
88.0 |
| QTPYAK37 |
1 |
70 |
Mms. luminyensis |
88.3 |
QTPC37 |
3 |
16 |
Mms. luminyensis |
86.6 |
| QTPYAK38 |
1 |
77 |
Mms. luminyensis |
87.9 |
QTPC38 |
5 |
39 |
Mms. luminyensis |
86.6 |
| QTPYAK39 |
3 |
70 |
Mms. luminyensis |
88.5 |
QTPC39 |
9 |
39 |
Mms. luminyensis |
86.5 |
| QTPYAK40 |
1 |
70 |
Mms. luminyensis |
88.4 |
QTPC40 |
2 |
39 |
Mms. luminyensis |
86.7 |
| QTPYAK41 |
1 |
70 |
Mms. luminyensis |
88.4 |
QTPC41 |
1 |
16 |
Mms. luminyensis |
86.5 |
| QTPYAK42 |
1 |
70 |
Mms. luminyensis |
88.6 |
QTPC42 |
3 |
58 |
Mms. luminyensis |
87.8 |
| QTPYAK43 |
4 |
74 |
Mms. luminyensis |
87.8 |
QTPC43 |
2 |
16 |
Mms. luminyensis |
86.7 |
| QTPYAK44 |
4 |
74 |
Mms. luminyensis |
87.9 |
QTPC44 |
3 |
58 |
Mms. luminyensis |
88.3 |
| QTPYAK45 |
2 |
74 |
Mms. luminyensis |
87.9 |
QTPC45 |
4 |
69 |
Mms. luminyensis |
86.6 |
| QTPYAK46 |
1 |
71 |
Mms. luminyensis |
88.6 |
QTPC46 |
1 |
56 |
Mms. luminyensis |
87.9 |
| QTPYAK47 |
7 |
81 |
Mmc. blatticola |
92.9 |
QTPC47 |
1 |
55 |
Mms. luminyensis |
87.9 |
| QTPYAK48 |
2 |
81 |
Mmc. blatticola |
92.9 |
QTPC48 |
1 |
67 |
Mms. luminyensis |
88.6 |
| QTPYAK49 |
1 |
81 |
Mmc. blatticola |
91.7 |
QTPC49 |
1 |
82 |
Mbb. millerae |
98.5 |
| QTPYAK50 |
2 |
81 |
Mmc. blatticola |
92.6 |
QTPC50 |
1 |
1 |
Mms. luminyensis |
87.6 |
| QTPYAK51 |
2 |
49 |
Mms. luminyensis |
88.7 |
QTPC51 |
1 |
82 |
Mbb. millerae |
97.5 |
| QTPYAK52 |
1 |
37 |
Mms. luminyensis |
88.0 |
QTPC52 |
1 |
82 |
Mbb. millerae |
98.3 |
| QTPYAK53 |
1 |
57 |
Mms. luminyensis |
87.7 |
QTPC53 |
1 |
82 |
Mbb. millerae |
98.2 |
| QTPYAK54 |
2 |
74 |
Mms. luminyensis |
87.9 |
QTPC55 |
1 |
82 |
Mbb. millerae |
98.8 |
| QTPYAK55 |
1 |
76 |
Mms. luminyensis |
87.1 |
QTPC56 |
1 |
25 |
Mms. luminyensis |
86.7 |
| QTPYAK56 |
2 |
72 |
Mms. luminyensis |
87.5 |
QTPC57 |
1 |
41 |
Mms. luminyensis |
86.4 |
| QTPYAK57 |
1 |
72 |
Mms. luminyensis |
87.6 |
QTPC58 |
2 |
94 |
Mbb. millerae |
96.0 |
| QTPYAK58 |
2 |
72 |
Mms. luminyensis |
87.9 |
QTPC59 |
2 |
55 |
Mms. luminyensis |
87.8 |
| QTPYAK59 |
1 |
75 |
Mms. luminyensis |
87.3 |
QTPC60 |
4 |
55 |
Mms. luminyensis |
87.7 |
| QTPYAK60 |
1 |
70 |
Mms. luminyensis |
88.1 |
QTPC61 |
2 |
55 |
Mms. luminyensis |
87.8 |
| QTPYAK61 |
1 |
39 |
Mms. luminyensis |
86.3 |
QTPC62 |
1 |
73 |
Mms. luminyensis |
87.6 |
| QTPYAK62 |
2 |
39 |
Mms. luminyensis |
86.2 |
QTPC63 |
1 |
41 |
Mms. luminyensis |
86.5 |
| QTPYAK63 |
2 |
39 |
Mms. luminyensis |
86.5 |
QTPC64 |
1 |
91 |
Mbb. millerae |
96.1 |
| QTPYAK64 |
4 |
46 |
Mms. luminyensis |
86.7 |
QTPC65 |
1 |
73 |
Mms. luminyensis |
87.5 |
| QTPYAK65 |
1 |
49 |
Mms. luminyensis |
88.4 |
QTPC66 |
1 |
40 |
Mms. luminyensis |
87.4 |
| QTPYAK67 |
2 |
80 |
Mmb. mobile |
99.8 |
QTPC68 |
1 |
7 |
Mms. luminyensis |
87.4 |
| QTPYAK68 |
1 |
64 |
Mms. luminyensis |
87.5 |
QTPC69 |
1 |
82 |
Mbb. millerae |
98.6 |
| QTPYAK69 |
2 |
93 |
Mbb. ruminantium |
96.7 |
QTPC70 |
1 |
94 |
Mbb. arboriphilus |
95.5 |
| QTPYAK70 |
1 |
87 |
Mbb. ruminantium |
96.8 |
QTPC71 |
1 |
59 |
Mms. luminyensis |
88.9 |
| QTPYAK71 |
1 |
87 |
Mbb. smithii |
96.5 |
QTPC72 |
1 |
59 |
Mms. luminyensis |
89.2 |
| QTPYAK72 |
1 |
32 |
Mms. luminyensis |
86.8 |
QTPC73 |
3 |
1 |
Mms. luminyensis |
87.8 |
| QTPYAK73 |
1 |
92 |
Mbb. ruminantium |
98.1 |
QTPC74 |
10 |
16 |
Mms. luminyensis |
86.6 |
| QTPYAK74 |
1 |
92 |
Mbb. ruminantium |
98.9 |
QTPC75 |
1 |
16 |
Mms. luminyensis |
86.5 |
| QTPYAK75 |
1 |
35 |
Mms. luminyensis |
87.2 |
QTPC76 |
2 |
16 |
Mms. luminyensis |
86.6 |
| QTPYAK76 |
1 |
49 |
Mms. luminyensis |
88.4 |
QTPC77 |
6 |
16 |
Mms. luminyensis |
86.6 |
| QTPYAK77 |
1 |
42 |
Mms. luminyensis |
88.3 |
QTPC78 |
1 |
16 |
Mms. luminyensis |
86.6 |
| QTPYAK78 |
1 |
42 |
Mms. luminyensis |
87.5 |
QTPC79 |
1 |
24 |
Mms. luminyensis |
87.0 |
| QTPYAK79 |
1 |
16 |
Mms. luminyensis |
86.6 |
QTPC80 |
1 |
16 |
Mms. luminyensis |
86.2 |
| QTPYAK80 |
1 |
16 |
Mms. luminyensis |
86.7 |
QTPC81 |
1 |
16 |
Mms. luminyensis |
86.7 |
| QTPYAK81 |
10 |
16 |
Mms. luminyensis |
86.6 |
QTPC82 |
1 |
20 |
Mms. luminyensis |
83.8 |
| QTPYAK82 |
1 |
16 |
Mms. luminyensis |
86.5 |
QTPC83 |
1 |
9 |
Mms. luminyensis |
87.6 |
| QTPYAK83 |
1 |
16 |
Mms. luminyensis |
86.4 |
QTPC84 |
1 |
24 |
Mms. luminyensis |
86.4 |
| QTPYAK84 |
3 |
16 |
Mms. luminyensis |
86.4 |
QTPC85 |
1 |
26 |
Mms. luminyensis |
86.4 |
| QTPYAK85 |
1 |
16 |
Mms. luminyensis |
86.4 |
QTPC86 |
2 |
48 |
Mms. luminyensis |
87.3 |
| QTPYAK86 |
1 |
16 |
Mms. luminyensis |
86.7 |
QTPC87 |
1 |
21 |
Mms. luminyensis |
86.8 |
| QTPYAK87 |
1 |
16 |
Mms. luminyensis |
86.7 |
QTPC88 |
1 |
23 |
Mms. luminyensis |
86.3 |
| QTPYAK88 |
1 |
16 |
Mms. luminyensis |
87.0 |
QTPC89 |
1 |
22 |
Mms. luminyensis |
86.4 |
| QTPYAK89 |
1 |
16 |
Mms. luminyensis |
86.6 |
QTPC90 |
1 |
39 |
Mms. luminyensis |
87.3 |
| QTPYAK90 |
1 |
16 |
Mms. luminyensis |
86.7 |
QTPC91 |
1 |
42 |
Mms. luminyensis |
87.9 |
| QTPYAK91 |
2 |
16 |
Mms. luminyensis |
86.5 |
QTPC92 |
1 |
2 |
Mms. luminyensis |
86.9 |
| QTPYAK92 |
1 |
16 |
Mms. luminyensis |
87.4 |
QTPC93 |
1 |
2 |
Mms. luminyensis |
88.0 |
| QTPYAK93 |
1 |
16 |
Mms. luminyensis |
87.2 |
QTPC94 |
1 |
1 |
Mms. luminyensis |
87.7 |
| QTPYAK94 |
6 |
16 |
Mms. luminyensis |
86.5 |
QTPC95 |
6 |
81 |
Mmc. blatticola |
92.8 |
| QTPYAK95 |
2 |
16 |
Mms. luminyensis |
86.3 |
QTPC96 |
6 |
81 |
Mmc. blatticola |
92.5 |
| QTPYAK96 |
2 |
16 |
Mms. luminyensis |
87.2 |
QTPC97 |
2 |
39 |
Mms. luminyensis |
87.1 |
| QTPYAK97 |
1 |
16 |
Mms. luminyensis |
86.3 |
QTPC98 |
1 |
39 |
Mms. luminyensis |
87.2 |
| QTPYAK98 |
1 |
15 |
Mms. luminyensis |
87.2 |
QTPC99 |
1 |
47 |
Mms. luminyensis |
86.4 |
| QTPYAK99 |
1 |
27 |
Mms. luminyensis |
87.1 |
QTPC100 |
1 |
59 |
Mms. luminyensis |
88.5 |
| QTPYAK100 |
1 |
27 |
Mms. luminyensis |
87.4 |
QTPC101 |
1 |
79 |
Mms. luminyensis |
87.1 |
| QTPYAK101 |
1 |
14 |
Mms. luminyensis |
87.0 |
QTPC102 |
1 |
5 |
Mms. luminyensis |
88.4 |
| QTPYAK102 |
1 |
24 |
Mms. luminyensis |
86.7 |
QTPC103 |
1 |
6 |
Mms. luminyensis |
87.6 |
| QTPYAK103 |
1 |
12 |
Mms. luminyensis |
87.3 |
QTPC104 |
1 |
66 |
Mms. luminyensis |
88.5 |
| QTPYAK104 |
1 |
19 |
Mms. luminyensis |
85.5 |
QTPC105 |
1 |
29 |
Mms. luminyensis |
86.4 |
| QTPYAK105 |
1 |
13 |
Mms. luminyensis |
87.5 |
QTPC106 |
1 |
45 |
Mms. luminyensis |
87.4 |
| QTPYAK106 |
1 |
17 |
Mms. luminyensis |
85.9 |
QTPC107 |
1 |
54 |
Mms. luminyensis |
87.7 |
| QTPYAK107 |
1 |
17 |
Mms. luminyensis |
86.4 |
QTPC108 |
1 |
48 |
Mms. luminyensis |
86.7 |
| QTPYAK108 |
1 |
11 |
Mms. luminyensis |
86.8 |
QTPC109 |
1 |
30 |
Mms. luminyensis |
86.5 |
| QTPYAK109 |
3 |
16 |
Mms. luminyensis |
86.5 |
QTPC110 |
1 |
95 |
Mbb. wolinii |
95.7 |
| QTPYAK110 |
1 |
18 |
Mms. luminyensis |
86.2 |
QTPC111 |
1 |
39 |
Mms. luminyensis |
86.3 |
| QTPYAK111 |
1 |
16 |
Mms. luminyensis |
86.8 |
QTPC112 |
1 |
92 |
Mbb. ruminantium |
99.0 |
| QTPYAK112 |
2 |
16 |
Mms. luminyensis |
85.9 |
QTPC113 |
1 |
43 |
Mms. luminyensis |
88.4 |
| QTPYAK113 |
1 |
18 |
Mms. luminyensis |
86.3 |
QTPC114 |
1 |
42 |
Mms. luminyensis |
87.7 |
| QTPYAK114 |
2 |
16 |
Mms. luminyensis |
86.2 |
|
|
|
|
|
| QTPYAK115 |
1 |
16 |
Mms. luminyensis |
86.3 |
|
|
|
|
|
| QTPYAK116 |
1 |
34 |
Mms. luminyensis |
87.2 |
|
|
|
|
|
| QTPYAK117 |
2 |
34 |
Mms. luminyensis |
87.7 |
|
|
|
|
|
| QTPYAK118 |
1 |
8 |
Mms. luminyensis |
88.1 |
|
|
|
|
|
| QTPYAK119 |
2 |
34 |
Mms. luminyensis |
87.9 |
|
|
|
|
|
| QTPYAK120 |
1 |
41 |
Mms. luminyensis |
86.3 |
|
|
|
|
|
| QTPYAK121 |
1 |
89 |
Mbb. smithii |
96.2 |
|
|
|
|
|
| QTPYAK122 |
1 |
44 |
Mms. luminyensis |
87.9 |
|
|
|
|
|
| QTPYAK123 |
1 |
58 |
Mms. luminyensis |
87.9 |
|
|
|
|
|
| QTPYAK124 |
1 |
78 |
Mms. luminyensis |
88.1 |
|
|
|
|
|
| QTPYAK125 |
1 |
59 |
Mms. luminyensis |
89.1 |
|
|
|
|
|
| QTPYAK126 |
1 |
59 |
Mms. luminyensis |
89.2 |
|
|
|
|
|
| QTPYAK127 |
1 |
74 |
Mms. luminyensis |
88.1 |
|
|
|
|
|
| QTPYAK128 |
1 |
2 |
Mms. luminyensis |
87.7 |
|
|
|
|
|
| QTPYAK129 |
2 |
38 |
Mms. luminyensis |
88.2 |
|
|
|
|
|
| QTPYAK130 |
1 |
65 |
Mms. luminyensis |
88.7 |
|
|
|
|
|
| QTPYAK132 |
1 |
58 |
Mms. luminyensis |
88.9 |
|
|
|
|
|
| QTPYAK133 |
1 |
60 |
Mms. luminyensis |
88.7 |
|
|
|
|
|
| QTPYAK134 |
1 |
2 |
Mms. luminyensis |
87.3 |
|
|
|
|
|
| QTPYAK135 | 1 | 21 | Mms. luminyensis | 87.1 | |||||
Mbb.= Methanobrevibacter; Mms=Methanomassiliicoccus; Mmb=Methanomicrobium; Mmc=Methanimicrococcus.
*16S Sequences were obtained from MOTHUR program as unique sequences, while OTUs were generated by the MOTHUR program at 98% species level identity.