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Total RNA was isolated following the incubation in water or in 100 mm mannitol (Man), 3-o-methylglucose (OMG), Glc, Fru, or Suc for 15 h. After quantifying abundance of mRNA with a phosphor imager, the x-ray film was exposed for 12 and 2 d, respectively, for Frk1 and Frk2.
