Table 2.
Oligonucleotides used in this study
| Oligo name | Note | Sequence (5′– 3′) |
|---|---|---|
| MDS-4 |
Reverse transcription |
CGCTCTTCCGATCTNNNNNN |
| MDS-189 |
Library amplification |
CTGTCTGGCTCTTCCGATCT |
| MDS-115 |
Degenerate astrovirus consensus |
CCATCAGGTCARWWNTCAACAAC |
| MDS-116 |
Degenerate astrovirus consensus |
CTCGCTAATHTAYGGDGATGA |
| MDS-117 |
Degenerate astrovirus consensus |
GGTTTNACCCACATNCCAAA |
| MDS-118 |
Degenerate astrovirus consensus |
GTCCAACTGTGADNCCACARAA |
| MDS-119 |
Rabbit astrovirus diagnostic |
ATAATAACATGGTCAACTATTGGCTTC |
| MDS-120 |
Rabbit astrovirus diagnostic |
GACATCCTTATACATTTTCACAACTTT |
| MDS-156 |
Vertebrate rRNA (L2513) |
GCCTGTTTACCAAAAACATCAC |
| MDS-157 |
Vertebrate rRNA (H2714) |
CTCCATAGGGTCTTCTCGTCTT |
| MDS-298 |
Genome recovery and sequencing (27–46 forward) |
CGGCCAGAGAGCCTATTACC |
| MDS-296 |
5′ RACE (149–168 reverse) |
GGCTAGAGGAATGGGGTCAG |
| MDS-299 |
Genome recovery and sequencing (1146–1165 forward) |
GCTCGTTCCGATAATTGCTC |
| MDS-300 |
Genome recovery and sequencing (1229–1248 reverse) |
GCAACTAACCACGCACAATG |
| MDS-144 |
Genome recovery and sequencing (2554–2564 forward) |
TCTGGACYGARGARGARTA |
| MDS-300 |
Genome recovery and sequencing (2639–2659 reverse) |
CACTCCATTCAGGGTAACCAA |
| MDS-135 |
Genome recovery and sequencing (3573–3592 forward) |
TCCACTCCCGCCTACCCCAA |
| MDS-145 |
Genome recovery and sequencing (3622–3641 reverse) |
CCACCCACAATCAGAGAGGT |
| MDS-119 |
Genome recovery and sequencing (4165–4191 forward) |
ATAATAACATGGTCAACTATTGGCTTC |
| MDS-125 |
Genome recovery and sequencing (4287–4308 reverse) |
CATAATCAGATGGGAGGACAGG |
| MDS-147 |
Genome recovery and sequencing (4602–4620 forward) |
CTTCGCAGCCACTCTCTTG |
| MDS-137 |
Genome recovery and sequencing (4668–4683 reverse) |
TTGGTCCTCCCCTCCA |
| MDS-138 |
3′ RACE (5719–5729 forward) |
TGGTGGTTTGT |
| MDS-150 |
Genome recovery and sequencing (5759–5779 reverse) |
TCTGAAATTGCACTGTGTTGG |
| MDS-121 |
RACE oligo-dT primer |
CCAGTGAGCAGAGTGACGAGGACTCGAGCTCAAGCT17 |
| MDS-122 |
RACE outer adapter primer |
CCAGTGAGCAGAGTGACG |
| MDS-123 | RACE inner adapter primer | GAGGACTCGAGCTCAAGC |