Table 1. Fluorescence decay fitting parameters (τ1 and τ2) of 5 µM SG in the absence and presence of 5 µM DNAsΔ.
τ1 (ns) | τ2 (ns) | χ2 | |
DNA free | 3.20a | 1.047 | |
2.45b | 1.029 | ||
DNA1-A | 3.32a | 1.048 | |
2.90b (8.12%) | 12.05 b (91.88%) | 1.123 | |
DNA1-C | 2.76 a (74.27%) | 11.65 a (25.73%) | 1.003 |
14.05 b | 1.032 | ||
DNA1-G | 3.30 a | 1.067 | |
2.28 b (16.34%) | 11.75 b (83.66%) | 1.006 | |
DNA1-T | 2.87 a (76.62%) | 10.29 a (23.38%) | 1.019 |
13.61 b | 1.039 | ||
FM-DNA | 3.25 a | 1.063 | |
2.02 b (72.47%) | 7.52 b (27.53%) | 1.114 |
The lifetimes were measured at 415 nm (a) and 586 nm (b) with excitation at 336 nm. The lifetime measurement was not applicable for DNA3-Ys and DNA4-Ys due to the strong fluorescence quenching.