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. 2012 Nov 20;7(11):e48251. doi: 10.1371/journal.pone.0048251

Table 1. Fluorescence decay fitting parameters (τ1 and τ2) of 5 µM SG in the absence and presence of 5 µM DNAsΔ.

τ1 (ns) τ2 (ns) χ2
DNA free 3.20a 1.047
2.45b 1.029
DNA1-A 3.32a 1.048
2.90b (8.12%) 12.05 b (91.88%) 1.123
DNA1-C 2.76 a (74.27%) 11.65 a (25.73%) 1.003
14.05 b 1.032
DNA1-G 3.30 a 1.067
2.28 b (16.34%) 11.75 b (83.66%) 1.006
DNA1-T 2.87 a (76.62%) 10.29 a (23.38%) 1.019
13.61 b 1.039
FM-DNA 3.25 a 1.063
2.02 b (72.47%) 7.52 b (27.53%) 1.114
Δ

The lifetimes were measured at 415 nm (a) and 586 nm (b) with excitation at 336 nm. The lifetime measurement was not applicable for DNA3-Ys and DNA4-Ys due to the strong fluorescence quenching.