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. 2012 Nov 20;7(11):e49615. doi: 10.1371/journal.pone.0049615

Figure 4. RIN3 promotes RAB5 activation. (.

Figure 4

A) LAD2 cells transfected with control or RIN3 siRNA were stimulated with 100 ng/ml SCF for 15 minutes. Cells were lysed and activated RAB5 was pulled down using 4X-ZFYVE-GST on glutathione beads as indicated in Methods. Precipitates from the beads were immunoblotted for RAB5 (RAB5GTP). The whole cell lysates were immunoblotted for total RAB5, RIN3, pERK (to show stimulation), ERK, and TUBB (loading control). Graph represents data from three independent experiments, **p<0.01. (B) Cells infected with Ad-GFP or Ad-RIN3 and treated as in (A). Graph represents data from three independent experiments, ***p<1 × 10−5.