Figure 1. Effect of rapamycin on SCC tumor growth and mTOR signaling pathway.

(A) Tumor sizes of the SCC tumors in the mice leg treated with vehicle (control, •), 5 mg/kg bw/day (▴), and 10 mg/kg bw/day (▪) rapamycin. (B) Western blot analysis of S6 protein expression and the abundance of its phosphorylated form in SCC tumor cells treated with rapamycin (100 nM). (C, D) Immunostaining of pS6 in SCC xenograft of control and rapamycin treated (10 mg/kg bw/day, 2 days).