Figure 6. Mitochondrial dynamics of HeLa cells with Drp1 wt and mutants and were treated with LiCl and H89.

(A) HeLa cells were transfected with GFP-tagged Drp1wt or other mutants for 24 hours. Then cells were treated with 10 mM LiCl and 10 µM H89 for another 24 hours. Mitochondrial morphology of HeLa cells was observed by staining with Mitotracker under confocal microscopy. Cell nuclei were counter-stained by using DAPI. Insets are magnifications of the Mitotracker signal at the indicated areas. Inset 1 represents the non-transfected cells, and inset 2 indicates the transfected cells. Indications (white arrows) represent typical elongated mitochondria morphology. (B) Statistical result of mitochondrial morphology. After 24 hours treated with inhibitors (upper: LiCl, lower: H89), over 100 transfected cells were categorized into 3 groups depending on mitochondrial morphology. *p<0.05, **p<0.01, ***p<0.001.