Table 1.
Ga-peptide complexes | m/za | m/zcalculated as [M + H]+ | AnalyticalHPLC retention (min)b | Affinity-related IC50determined in enzyme-based assay (nM)c | Affinity-related IC50determined in cell-based assay (nM)c |
---|---|---|---|---|---|
[Ga]6 |
947.4257 |
947.4250 |
3.1 |
9.0 ± 1.1 |
12.1 ± 2.1 |
[Ga]7 | 1,361.6387 | 1,361.6364 | 3.1 | 2.1 ± 1.4 | 3.8 ± 1.8 |
aHigh-resolution mass spectrometry data of the free ligands ([M + H]+); bcompounds were labeled with 68Ga; runs were performed using a linear A-B gradient (0% B to 100% B in 6 min) at a flow rate of 4 mL/min; solvent A was 0.1% aqueous TFA, and solvent B was MeOH; ccompounds were complexed with natGa.