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MSP analysis of the four SFRP genes in MPN-derived cell lines and normal peripheral blood (PB).In vitro methylated DNA (IVD) and water served as controls. Lane U, amplified product with primers recognizing unmethylated SFRP-1, -2, -4, and -5 sequences. Lane M, amplified product with primers recognizing methylated SFRP-1, -2, -4, and -5 sequences.
