Fig. 7.

A, time courses of the reactions of the I1131C CFTR with 1 mM MTSES⁻ in the presence and absence of 10 μM GlyH-101. Data points represent mean ± S.E.M. (n = 3). Covalent labeling of the I1131C CFTR with MTSES⁻ resulted in increases in conductance. The lines represent the best fits of single-exponential functions to the conductance data. The half-life of each single-exponential fit is shown. The second-order reaction rate constants in the presence and absence of 10 μM GlyH-101 were 28 and 20 M⁻¹ s⁻¹, respectively. The EC₅₀ at 0 mV for GlyH-101 blockade for the I1131C CFTR was 0.86 ± 0.016 μM (n = 3). B, time courses of the reactions of the S341C CFTR with 1 mM NEM in the presence and absence of 10 μM GlyH-101. Data points represent mean ± S.E.M. (n = 3). Covalent labeling of the S341C CFTR with NEM resulted in reductions in conductance. The lines represent the best fits of single-exponential functions to the conductance data. The half-life of each single-exponential fit is shown. The second-order reaction rate constants in the presence and absence of 10 μM GlyH-101 were 5.3 and 16 M⁻¹ s⁻¹, respectively. The EC₅₀ at 0 mV for GlyH-101 blockade for the S341C CFTR was 0.89 ± 0.056 μM (n = 3).