Fig. 2.

The C-terminal domain between residues Y295 and Q314 plays an essential role in the regulation of HCA₂ export from the endoplasmic reticulum to the cell surface. a, HEK-293 cells stably expressing EGFP-tagged wild type HCA₂ (HCA₂-EGFP) or Δ295–314 (Δ295–314-EGFP) were stimulated with 200 μM niacin for 40 min. b, HEK-293 cells were transiently cotransfected with HCA₂-EGFP or Δ295–314-EGFP (green) and with pDsRed2-ER (red). After 24 h, cells were fixed and examined by confocal microscopy as described under Materials and Methods. All images are representative of at least three independent experiments.