Table 2.

Proteins significantly altered expression in DEDC exposure group vs. controls identified using pH 7–11 isoelectric focusing.

Average ratio DEDC Level vs. controlsa	t-testb(p <)	Accession No.c	Protein identificationd	Protein
−1.94	.01	ECHA_RAT Q64428		Hydroxyacyl-Coenzyme Adehydrogenase
−1.91	.01		77,15(l),0,23%
−1.79	.01		56,12(1),2,12%
−2.01	.01
1.22	.05	CN37_RAT P13233	243,11,9,23%	2',3'-cyclic-nucleotide 3'-phosphodiesterase-Rattus norvegicus
1.3	.05		288,16,7,30%
1.33	.05		249,17,6,33%
1.25	.05		120,12,4,23%
1.32	.05	DHPR_RAT P11348	86,6,3,18%	Dihydropteridine reductase- Rattus norvegicus
1.33	.05	GSTP1_RAT P04906	71,7,1,30%	Glutathione S-transferase P- Rattus norvegicus
1.38	.05	MBP_RAT P02688	273,16,7,59%	Myelin basic protein S- Rattus norvegicus
1.4	.05		145,12,3,48%

^aaverage volume ratios for each protein spot identified

^blevel of significance determined by students t-test using DeCyder software version 6.5 using the mixed sample internal standard methodology

^cSwiss-Prot database

^dImported from MASCOT and GPS Explorer. Data include predicted MW and pi to correlate with gel position (see supplementary material for annotated gel images), and the additional four scores: the four numbers associated with the protein identifications: e.g., 95¹ ,16(1)² ,3³ ,31%⁴ represent:

¹MOWSE score. MOlecular Weight SEarch score, a statistic calculated by the MASCOT database search algorithm. The 95th percentile cutoff is calculated for each search and indicated at the bottom of each analysis summary. About 5% of random matches could yield higher MOWSE scores. See matrixscience.com for more details.

²peptide #. number of peptide ion masses matched (number of un-matched ions).

³ms2 #. number of matching peptide ions with MS/MS data.

⁴(%coverage). Percent of the amino acids accounted for by the matching peptides