Fig. 3.
The selectivity for acetylated H3 is conserved. (a) Superimposed 1H,15N HSQC spectra of MOZ PHD1/2, recorded during addition of the indicated H3 peptides. The spectra are color-coded according to the protein-peptide ratio. (b) Binding affinities of the MOZ PHD1/2 fingers for indicated H3 peptides were measured by tryptophan fluorescence. (c) Western blot of bound MOZ PHD1/2 (beads) following in-solution pulldowns with the indicated peptides. Control shows detection of unbound protein following incubation with peptide-bead conjugate.