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. Author manuscript; available in PMC: 2013 Dec 14.
Published in final edited form as: J Mol Biol. 2012 Oct 12;424(5):328–338. doi: 10.1016/j.jmb.2012.10.004

Fig. 5.

Fig. 5

The PHD1/2 fingers of MORF are required for the interaction with histone H3 in cells. (a) IPs of the Flag-MORF [N-terminal region of MORF (N-MORF, amino acids 1–352) and N-MORFΔPHD1/2 (amino acids 1–215), in which the PHD1/2 fingers are deleted] from HEK293 cells and immunoblotting with indicated antibodies. (b) IPs of the Flag-N-MORF from HEK293 cells +/− TSA and immunoblotting with indicated antibodies. (c) HEK293 cells were transfected with indicated GFP-MORF constructs, and subcellular localization of the GFP signal was detected by live green fluorescence microscopy. (d, e) HAT assays with indicated substrates and MOZ-BRPF1-ING5-hEaf6 complex purified from co-transfected cells were quantified by liquid scintillation (d) and gel fluorography (e).