Reconstitutable (PLGA36kDa)2-b-bPEI micelle-based pDNA polyplex: (a) schematic representation of the structures of (PLGA36kDa)2-b-bPEI25kDa micelles, micelle/pDNA complexes, and micelle/pDNA/bPEILMW complexes, (b) particle sizes and surface charges of micelle/pDNA complexes and micelle/pDNA/bPEI1.8kDa complexes before and after reconstitution, (c) in vitro transfection efficiency of fresh and reconstituted micelle/pDNA/bPEI1.8kDa complexes (1 μg of pDNA) in MCF7 cells (5×105 cells seeded), and (d) pDNA-dose dependent transfection efficiency of “reconstituted” micelle/pDNA/bPEI1.8kDa (WR 1) complexes in MCF7 cells. When using 1 μg of pDNA, its concentration was 0.5 μg/mL. [37] (Reproduced with permission)