Table 2.
MCF7 | MCF7/ADR-RES | MES-SA | ||
---|---|---|---|---|
PEI/pDNA-mediated transfection | Condition AB | 2-fold ↓ | 2.2-fold ↑ | 2-fold ↑ |
Condition A | 7.7-fold ↓ | 2.1-fold ↓ | 1.6-fold ↓ | |
Condition B | 1.6-fold ↑ | 1.9-fold ↑ | 2.6-fold ↑ | |
| ||||
PLL/pDNA-mediated transfection | Condition AB | 1.7-fold ↑ | 1.8-fold ↑ | 1.5-fold ↓ |
Condition A | 1-fold | 2.4-fold ↓ | 2.7-fold ↓ | |
Condition B | 1.6-fold ↑ | 1.6-fold ↑ | 1.2-fold ↑ |
↓ and ↑ indicate lower and higher, respectively. Condition A (4-hr transfection period at different pH values (pH 7.4, 7.0, 6.7, and 6.3) followed by a 44 hr incubation period fixed at pH 7.4), Condition B (4 hr transfection period at pH 7.4 followed by a 44-hr incubation period at different pH values (pH 7.4, 7.0, 6.7, and 6.3)), Condition AB (48-hr transfection period and incubation period both at different pH values (7.4, 7.0, 6.7, and 6.3)).