Table 2.

Summary of cell transfection enhancement or reduction with extracellular pH 6.3 compared to extracellular pH 7.4. [30] (Reproduced with permission)

		MCF7	MCF7/ADR-RES	MES-SA
PEI/pDNA-mediated transfection	Condition AB	2-fold ↓	2.2-fold ↑	2-fold ↑
	Condition A	7.7-fold ↓	2.1-fold ↓	1.6-fold ↓
	Condition B	1.6-fold ↑	1.9-fold ↑	2.6-fold ↑
PLL/pDNA-mediated transfection	Condition AB	1.7-fold ↑	1.8-fold ↑	1.5-fold ↓
	Condition A	1-fold	2.4-fold ↓	2.7-fold ↓
	Condition B	1.6-fold ↑	1.6-fold ↑	1.2-fold ↑

↓ and ↑ indicate lower and higher, respectively. Condition A (4-hr transfection period at different pH values (pH 7.4, 7.0, 6.7, and 6.3) followed by a 44 hr incubation period fixed at pH 7.4), Condition B (4 hr transfection period at pH 7.4 followed by a 44-hr incubation period at different pH values (pH 7.4, 7.0, 6.7, and 6.3)), Condition AB (48-hr transfection period and incubation period both at different pH values (7.4, 7.0, 6.7, and 6.3)).