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. Author manuscript; available in PMC: 2013 Nov 19.
Published in final edited form as: Chem Res Toxicol. 2012 Oct 31;25(11):2462–2468. doi: 10.1021/tx3003033

Figure 2. Relative NER efficiencies of dG AF and dG AAF adducts in the NarI sequence.

Figure 2

A. Plasmids containing site-specific dG-AF (lane 1–3) or dG-AAF (lane 4–6) adducts were incubated with HeLa whole cell extracts. The excision products were detected by annealing to complementary oligonucleotides with a 4G overhang, which served as a template for end-labeling with [α-32P] dCTP with sequenase. The reaction products were resolved on a 14% denaturing polyacrylamide gel. The position of 25nt and 34nt markers is indicated on the left of the gel. B. Quantification of the relative NER efficiencies from at least three independent experiments.

Yeo et al.,