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. Author manuscript; available in PMC: 2013 Nov 19.
Published in final edited form as: Chem Res Toxicol. 2012 Oct 31;25(11):2462–2468. doi: 10.1021/tx3003033

Figure 3. Binding affinities of XPC RAD23B bound to dG AF and dG AAF substrates correlates with NER efficiency.

Figure 3

A. 44mer oligonucleotides containing a dG-AF or dG-AAF were annealed to complementary oligonucleotides containing a fluorescent label (5'-Cy5). The substrates were incubated with the indicated concentrations of XPC-RAD23B and the mixtures analyzed on to a 5% native polyacrylamide gel. The positions of the unbound DNA (D) and XPC-RAD23B-bound DNA (XD) are indicated. B. The binding data of three independent experiments were quantified by fitting to a sigmoidal curve and Kd values calculated using the Hill equation.

Yeo et al.,