Table 2.
Tm values and thermodynamic properties of dG-AF and dG-AAF containing duplexes.
| Substrate | Tmb (°C) | ΔH (kcal/mol) | ΔS (cal/mol•k) | ΔG (kcal/mol)c |
|---|---|---|---|---|
| dG (control) | 66 ± 1 | −102 ± 1 | −278 ± 1 | −15.8 ± 0.4 |
| dG1-AF | 55 ± 0 | −110 ± 0 | −310 ± 0 | −13.9 ± 0.1 |
| dG2-AF | 56 ± 0 | −105 ± 0 | −294 ± 1 | −13.8 ± 0.1 |
| dG3-AF | 51 ± 1 | −123 ± 1 | −354 ± 1 | −13.2 ± 0.4 |
| dG1-AAF | 54 ± 1 | −91 ± 1 | −254 ± 1 | −12.2 ± 0.3 |
| dG2-AAF | 50 ± 1 | −113 ± 1 | −325 ± 1 | −12.2 ± 0.3 |
| dGb-AAF | 48 ± 1 | −65 ± 2 | −178 ± 1 | −9.8 ± 0.2 |
a
Thermodynamic parameters for oligonucleotides containing modifications were acquired from absorbance versus temperature melting curves.
b
Melting temperatures were measured at an oligonucleotide concentration of 5.8 μM.
c
Gibbs free energy calculated at 37°C. Reported errors are standard deviations rounded to the next integral.