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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Nanomedicine. 2012 Jun 9;9(2):194–201. doi: 10.1016/j.nano.2012.05.015

Figure 3.

Figure 3

Flow cytometric analysis of T6-17 cells incubated with 50nm HER2-SPIO conjugates. (A) T6-17 cells were incubated with 50nm HER2-SPIO conjugates, with ligand densities ranging from 16.5 to 129 affibodies per SPIO, for 30 minutes at 37°C. Cell samples were then evaluated by flow cytometry. Each measurement represents the normalized mean fluorescence intensity (MFI). Statistically significant (p<0.05) improvements in cell binding were found to occur for targeted SPIO NPs with an intermediate ligand density (57.4 Affibody/SPIO). (B) The effect of differing ligand densities (HER2-AzFP per surface area) on cell targeting was compared for 26nm and 50nm SPIO. Despite the differences in nanoparticle hydrodynamic diameter, optimized cell targeting occurs at near identical ligand surface densities.