Table 1.
Assignments of m/z values detected in the negative ion mode MALDI-TOF mass spectra and PSD analyses of the P. furiosus total lipid extract to various lipid components. Lipids are indicated as abbreviations.
| Observed [M–H]− signals (m/z) | Assignments | Calculated [M–H]− (m/z) |
|---|---|---|
| 1784.6 | (Hexose-P)2-T | 1784.3 |
| 1741.5 | N-Acetyl-hexose-hexose-P-Tcyclic | 1741.4 |
| 1701.9 | Hexose2-P-T cyclic | 1702.4 |
| 1543.5 | BPG plus Na+ | 1542.3 |
| 1521.3 | BPG | 1520.3 |
| 976.2 | DGD | 975.8 |
| 934.8 | N-Acetyl-hexose-P-D | 934.7 |
| 922.6 | uns-N-Acetyl-hexose-P-D | 922.6 |
| 893.8 | PI | 893.7 |
| 881.6 | unsPI | 881.5 |
| 867.2 | PGP minus H2O | 868.6 |
| 814.4 | MGD | 813.7 |
| 805.7 | PG | 805.7 |
| 787.5 | PG minus H2O | 787.7 |
| 731.8 | PA | 731.6 |
| 241.3 | Hexose-P minus H2O | 242.0 |
Abbreviations—D: diether or archaeol; T: tetraether or caldarchaeol; Tcyclic: caldarchaeol with cyclopentane rings; P: phosphate group; uns: unsaturated branched-chains, 3 double bonds for chain; N-acetyl-hexose: N-acetylglucosamine; PI: diphytanylglycerol analogue of phosphatidylinositol; BPG: diphytanylglycerol analogue of bisphosphatidylglycerol (or ether lipid cardiolipin); PG: diphytanylglycerol analogue of phosphatidylglycerol; PGP: diphytanylglycerol analogue of phosphatidylglycerol-phosphate; PA: diphytanylglycerol analogue of phosphatidic acid; MGD: monoglycosyl-diphytanylglycerol; DGD: diglycosyl-diphytanylglycerol.