Fig. 3.

Receptor specific and dose dependent inhibition of Notch signalling between cells. Luciferase based co-culture assays were conducted with cells expressing the ligand Jagged1 and cells expressing different full-length Notch receptors (HEK-Notch1, HEK-Notch2 and HEK-Notch3). (A) Samples tested were anti-Notch1 antibody N1_E6, anti-Notch2 antibodies N2_B6 and N2_B9, the anti-human Notch3 antibody N3_mAb. Controls are non-activated, Jag1(-), and untreated, Jag1(+), cultures. The ratio of Firefly/Renilla luciferase activity is expressed relative to that of a control antibody (Shc1). (B) Dose response curves were generated by titration of N1_E6 on HEK-Notch1 and antibodies N2_B6 and N2_B9 on HEK-Notch2. The relative luciferase activity in non-activated cells were used to reflect 100% inhibition and the standard deviations were calculated to be 100 ± 6,65% for HEK-Notch1 (D) and 100 ± 2,51% for HEK-Notch2.