Fig. (2).

Analysis of purified recombinant proteins by metal chelating chromatography through SDS-12% PAGE. Purified protein eluted from Ni⁺² - charged chelating sepharose column with 1 M imidazole. Lane M, molecular mass protein marker; lane 1, rLIC10821 (37 kDa); lane 2, Lsa66 (65 kDa); lane 3, Lsa25 (24 kDa); lane 4, Lsa21 (21 kDa); lane 5, rLIC11030 (37 kDa); lane 6, rLIC10672 (23 kDa). Protein bands were visualized by Coomassie blue staining. Positions of molecular mass standards are indicated on the left (in kDa). (*) denotes each protein band.