Fig. (3).

Induction of humoral immune response in hamsters. The animals were immunized subcutaneously with PBS (negative control), Lsa21, Lsa66, rLIC11030, rLIC10821, Lsa25 or rLIC10672, 50 µg each and boostered after two weeks. Animals were bled on day 30 (prior challenge) and 51 (after the challenge) to determine antigen specific total IgG responses in sera of immunized groups by ELISA method. Microdilution plates were coated with recombinant proteins and incubated with a serial dilution of serum from the immunized hamsters for IgG measurements. The data represent a pooled serum from two hamsters per group (days 30) or survival animals after challenge (day 51). All antibody titers refer to challenge assay 1.